The imaging of singlet oxygen in single cells

John W. Snyder; Zhan Gao; John D.C. Lambert; Peter R. Ogilby

doi:10.1117/12.590239

8 April 2005 The imaging of singlet oxygen in single cells

John W. Snyder, Zhan Gao, John D.C. Lambert, Peter R. Ogilby

Proceedings Volume 5689, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIV; (2005) https://doi.org/10.1117/12.590239
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

The lowest excited state of molecular oxygen, singlet molecular oxygen (a¹Δ_g), is generally regarded to be the active cytotoxic species in photodynamic therapy (PDT). As a result, the direct detection of singlet oxygen in biological systems should be of great value in elucidating the mechanisms underlying the observed effects of PDT. We have recently shown that singlet oxygen can be detected by its weak 1270 nm phosphorescence (a¹Δ_g→X³Σ_g^-) from a single nerve cell upon irradiation of a photosensitizer incorporated into the cell. In this paper, we discuss issues pertinent to the direct optical detection and imaging of singlet oxygen from single cells.

Citation Download Citation

John W. Snyder, Zhan Gao, John D.C. Lambert, and Peter R. Ogilby "The imaging of singlet oxygen in single cells", Proc. SPIE 5689, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIV, (8 April 2005); https://doi.org/10.1117/12.590239

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