The adaptive BioCD: interferometric immunoassay on a spinning disk

Leilei Peng; Manoj M. Varma; Fred E. Regnier; David D. Nolte

doi:10.1117/12.589217

1 April 2005 The adaptive BioCD: interferometric immunoassay on a spinning disk

Leilei Peng, Manoj M. Varma, Fred E. Regnier, David D. Nolte

Proceedings Volume 5692, Advanced Biomedical and Clinical Diagnostic Systems III; (2005) https://doi.org/10.1117/12.589217
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

The speed of interferometric detection is at least 1000 times faster than the fluorometric detection used in the vast majority of clinical diagnostic systems. This opens the possibility to perform thousands of assays in the time it takes fluorescence to perform only one. Molecules immobilized on a spinning disk, like a CD, present the fastest and simplest means of interrogating thousands of micron-scale interferometer elements per second. However, the challenge of interferometry on a spinning disk is to maintain stable phase in the presence of mechanical vibration. In this paper, we demonstrate the first use of adaptive optics in an adaptive optical homodyne mixer to perform interferometry on a multi-analyte BioCD. The BioCD is a 4" diameter glass disk printed with a spoke pattern of protein. When the disk spins, the periodic protein pattern is transferred into a high-speed optical phase modulation by spinning the disk at 3000 rpm in the path of a probe laser. A nonlinear optical film mixes the signal beam with a stable reference beam in a two-wave mixing configuration that adaptively phase-locks the two beams to create stable phase in spite of mechanical vibration. Specific binding of antibody to printed antigen is detected as an increased homodyne signal. Multi-analyte detection on Anti Mouse and Anti Rabbit IgG is performed in which Mouse IgG and Rabbit IgG act as the non-specific reagent to each other. Detection is made on circular tracks. The technique has the potential of fast screening for large numbers of protein interactions.

Citation Download Citation

Leilei Peng, Manoj M. Varma, Fred E. Regnier, and David D. Nolte "The adaptive BioCD: interferometric immunoassay on a spinning disk", Proc. SPIE 5692, Advanced Biomedical and Clinical Diagnostic Systems III, (1 April 2005); https://doi.org/10.1117/12.589217

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available