23 March 2005 AOTF-based system for image cytometry
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Abstract
This paper describes a simple, inexpensive multispectral imaging system for image cytometry applications. The system is based on an acousto-optical tunable filter (AOTF), a monochrome CCD camera, and a research-grade fluorescence microscope. The selected TeO2 AOTF has a 10x10 mm2 entrance aperture and operates within the spectral range of 447-750 nm. The bandpass of the filter varies between 1.4 nm at 450 nm and 5.1 nm at 690 nm. The control software works within the environment of a popular image-acquisition and -processing package Image Pro-Plus, making this system easy to integrate with many existing fluorescence microscopes and cameras. Since image-cytometry applications do not require very high spatial resolution, AOTF-based systems may become an interesting alternative to more complex and expensive LCTF or pushbroom methods.
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Bartek Rajwa, Wamiq Ahmed, Murugesan Venkatapathi, Gerald Gregori, Feng Jin, Jolanta Soos, Sudhir Trivedi, J. Paul Robinson, "AOTF-based system for image cytometry", Proc. SPIE 5694, Spectral Imaging: Instrumentation, Applications, and Analysis III, (23 March 2005); doi: 10.1117/12.591115; https://doi.org/10.1117/12.591115
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