23 March 2005 AOTF-based system for image cytometry
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This paper describes a simple, inexpensive multispectral imaging system for image cytometry applications. The system is based on an acousto-optical tunable filter (AOTF), a monochrome CCD camera, and a research-grade fluorescence microscope. The selected TeO2 AOTF has a 10x10 mm2 entrance aperture and operates within the spectral range of 447-750 nm. The bandpass of the filter varies between 1.4 nm at 450 nm and 5.1 nm at 690 nm. The control software works within the environment of a popular image-acquisition and -processing package Image Pro-Plus, making this system easy to integrate with many existing fluorescence microscopes and cameras. Since image-cytometry applications do not require very high spatial resolution, AOTF-based systems may become an interesting alternative to more complex and expensive LCTF or pushbroom methods.
© (2005) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Bartek Rajwa, Bartek Rajwa, Wamiq Ahmed, Wamiq Ahmed, Murugesan Venkatapathi, Murugesan Venkatapathi, Gerald Gregori, Gerald Gregori, Feng Jin, Feng Jin, Jolanta Soos, Jolanta Soos, Sudhir Trivedi, Sudhir Trivedi, J. Paul Robinson, J. Paul Robinson, } "AOTF-based system for image cytometry", Proc. SPIE 5694, Spectral Imaging: Instrumentation, Applications, and Analysis III, (23 March 2005); doi: 10.1117/12.591115; https://doi.org/10.1117/12.591115


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