15 April 2005 FT-IR analysis of phosphorylation and laser-dephosphorylation process
Author Affiliations +
Abstract
Phosphorylation and dephosphorylation, which are the most remarkable posttranslational modifications, are considered to be important chemical reactions that control the activation of proteins. In regenerative medicine, it has been reported that phosphorylated proteins are instrumental in the calcification of osteoconnective tissue. We examine the phosphorylation analysis method by measuring the infrared absorption peak of phosphate group that observed at about 1070 cm-1 (9.4 mm) with Fourier Transform Infrared Spectrometer (FT-IR). This result indicates that it is possible to identify a phosphorylation by measuring the infrared absorption peak of phosphate group observed at about 1070 cm-1 with FT-IR method. And we examine laser-dephosphorylation using Free Electron Laser (FEL) as a novel dephosphorylation method. After irradiation of 9.4mm-FEL, infrared absorption peak of phosphate group is reduced. It is suspected that this lowering of the peak of phosphate group is the effect like dephosphorylation. These novel methods can be applied to quality control technology in regenerative medicine.
© (2005) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Katsunori Ishii, Katsunori Ishii, Kaoru Yanase, Kaoru Yanase, Sachiko Suzuki-Yoshihashi, Sachiko Suzuki-Yoshihashi, Masaya Yamamoto, Masaya Yamamoto, Kunihiro Chihara, Kunihiro Chihara, Yasuhiko Tabata, Yasuhiko Tabata, Kunio Awazu, Kunio Awazu, } "FT-IR analysis of phosphorylation and laser-dephosphorylation process", Proc. SPIE 5695, Optical Interactions with Tissue and Cells XVI, (15 April 2005); doi: 10.1117/12.591904; https://doi.org/10.1117/12.591904
PROCEEDINGS
6 PAGES


SHARE
Back to Top