15 April 2005 Multiphoton nanosurgery in cells and tissues
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Abstract
Multiphoton Microscopy with a femtosecond pulsed Ti:sapphire laser in the near infrared (NIR) enables the user not only to image cells and tissues with a subcellular resolution but also to perform highly precise nanosurgery. Intratissue compartments, single cells and even cell organelles like mitochondria, membranes or chromosomes can be manipulated and optically knocked out. Working at transient TW/cm2 laser intensities, single cells of tumor-sphaeroids were eliminated efficiently inside the sphaeroid without damaging the neighbour cells. Also single organelles of cells inside tissues could be optically knocked out with the nanoscalpel without collateral damage. Tissue structures inside a human tooth have been ablated with sizes below 1 μm. This method may become a useful instrument for nano-manipulating and surgery in several fields of science, including targeted transfection.
© (2005) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Iris Riemann, Tiemo Anhut, Frank Stracke, Ronan Le Harzic, Karsten Koenig, "Multiphoton nanosurgery in cells and tissues", Proc. SPIE 5695, Optical Interactions with Tissue and Cells XVI, (15 April 2005); doi: 10.1117/12.590487; https://doi.org/10.1117/12.590487
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