Enhancement of synaptic transmission induced by BDNF in cultured cortical neurons

Jun He; Hui Gong; Shaoqun Zeng; Yanling Li; Qingming Luo

doi:10.1117/12.592072

29 March 2005 Enhancement of synaptic transmission induced by BDNF in cultured cortical neurons

Jun He, Hui Gong, Shaoqun Zeng, Yanling Li, Qingming Luo

Proceedings Volume 5696, Complex Dynamics and Fluctuations in Biomedical Photonics II; (2005) https://doi.org/10.1117/12.592072
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Brain-derived neurotrophic factor (BDNF), like other neurotrophins, has long-term effects on neuronal survival and differentiation; furthermore, BDNF has been reported to exert an acute potentiation of synaptic activity and are critically involved in long-term potentiation (LTP). We found that BDNF rapidly induced potentiation of synaptic activity and an increase in the intracellular Ca²⁺ concentration in cultured cortical neurons. Within minutes of BDNF application to cultured cortical neurons, spontaneous firing rate was dramatically increased as were the frequency and amplitude of excitatory spontaneous postsynaptic currents (EPSCs). Fura-2 recordings showed that BDNF acutely elicited an increase in intracellular calcium concentration ([Ca²⁺]_c). This effect was partially dependent on [Ca²⁺]_o; The BDNF-induced increase in [Ca²⁺]_c can not be completely blocked by Ca²⁺-free solution. It was completely blocked by K252a and partially blocked by Cd²⁺ and TTX. The results demonstrate that BDNF can enhances synaptic transmission and that this effect is accompanied by a rise in [Ca²⁺]_c that requires two route: the release of Ca²⁺ from intracellular calcium stores and influx of extracellular Ca²⁺ through voltage-dependent Ca²⁺ channels in cultured cortical neurons.

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Jun He, Hui Gong, Shaoqun Zeng, Yanling Li, and Qingming Luo "Enhancement of synaptic transmission induced by BDNF in cultured cortical neurons", Proc. SPIE 5696, Complex Dynamics and Fluctuations in Biomedical Photonics II, (29 March 2005); https://doi.org/10.1117/12.592072

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KEYWORDS

Calcium

Neurons

Synaptic transmission

Receptors

Sodium

Cadmium

Luminescence

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