OCT assessment of aggregation and sedimentation in concentrated RBC suspension: comparison of experimental and Monte Carlo simulated data

Mikhail Yu. Kirillin; Alexander V Priezzhev; Valery V. Tuchin; Ruikang K. Wang; Risto Myllyla

doi:10.1117/12.596850

29 March 2005 OCT assessment of aggregation and sedimentation in concentrated RBC suspension: comparison of experimental and Monte Carlo simulated data

Mikhail Yu. Kirillin, Alexander V Priezzhev, Valery V. Tuchin, Ruikang K. Wang, Risto Myllyla

Author Affiliations +

Proceedings Volume 5696, Complex Dynamics and Fluctuations in Biomedical Photonics II; (2005) https://doi.org/10.1117/12.596850
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

In this work, we use Monte Carlo simulation to obtain model OCT signals from a horizontally orientated blood layer at different stages of red blood cell (RBC) aggregation and sedimentation processes. The parameters for aggregating and sedimenting blood cells were chosen basing on the data available from literature and our earlier experimental studies. Two different models of simulated medium are considered: a suspension of washed RBC in physiological solution (where the aggregation does not take place) and RBC in blood plasma (which provides necessary conditions for aggregation). Good agreement of the simulation results with the available experimental data shows that the chosen optical parameters are reasonable. Dependencies of the numbers of photons contributing to the OCT signal on the number of experienced scattering events were analyzed for each simulated signal. It was shown, that maxima on these dependencies correspond to the peaks in the OCT signals related to the interfaces between the layers of blood plasma and blood cells. Their positions can be calculated from the optical thicknesses of the layers, and the absorption and scattering coefficients of the media.

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Mikhail Yu. Kirillin, Alexander V Priezzhev, Valery V. Tuchin, Ruikang K. Wang, and Risto Myllyla "OCT assessment of aggregation and sedimentation in concentrated RBC suspension: comparison of experimental and Monte Carlo simulated data", Proc. SPIE 5696, Complex Dynamics and Fluctuations in Biomedical Photonics II, (29 March 2005); https://doi.org/10.1117/12.596850

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KEYWORDS

Blood

Monte Carlo methods

Optical coherence tomography

Scattering

Photons

Signal processing

Plasma

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