29 March 2005 Observations on the forward-scattered speckle patterns from healthy human fibroblasts and melanoma cells
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Abstract
Confluent monolayers of Malme-3M (ATCC HTB-64) cells, which is a melanoma cell line, and Malme-3 (ATCC HTB-102) cells, which are normal skin fibroblast cells, were cultured and plated onto glass coverslips. The two lines were isolated from the same patient, providing a tumor and normal counterpart for comparative studies. The forward-scattered objective speckle patterns were observed using a HeNe lasers that emitted at 543 and 633 nm. First and second-order speckle statistics from the two cell types were compared and contrasted. Of particular interest were the mean and maximum speckle size for a given observation geometry, degree of polarization, contrast, and the intensity probability distribution function. The speckle patterns from dry, densely-packed single layer latex sphere samples of various sizes were also investigated for comparison. The results indicate that the far-forward speckle patterns from these confluent monolayers can be used to discriminate between the cell types and can be used to derive specific morphological parameters of the cells.
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Sean J. Kirkpatrick, Sean J. Kirkpatrick, Leif Vigeland, Leif Vigeland, Donald D Duncan, Donald D Duncan, } "Observations on the forward-scattered speckle patterns from healthy human fibroblasts and melanoma cells", Proc. SPIE 5696, Complex Dynamics and Fluctuations in Biomedical Photonics II, (29 March 2005); doi: 10.1117/12.590422; https://doi.org/10.1117/12.590422
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