Two-dimensional thermal analysis for freezing of endothelial cells by high-speed microscopic IR focal plane arrays

Junko Morikawa; Toshimasa Hashimoto; Kimiko Yamamoto; Joji Ando

doi:10.1117/12.591527

25 April 2005 Two-dimensional thermal analysis for freezing of endothelial cells by high-speed microscopic IR focal plane arrays

Junko Morikawa, Toshimasa Hashimoto, Kimiko Yamamoto, Joji Ando

Author Affiliations +

Proceedings Volume 5697, Photons Plus Ultrasound: Imaging and Sensing 2005: The Sixth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics; (2005) https://doi.org/10.1117/12.591527
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Two-dimensional micro scale thermal analysis is proposed for the measurement of the latent heat released from the cultured vascular endothelial cells during freezing by use of high-speed infrared focal plane arrays. The thermo graphically observed latent heat spreading over the tissues and the inter- or intracellular thermal diffusion were detected with a spatial resolution of 7.5mm. The temperature distribution in time and space in each endothelial cell was influenced by both the self-release and intercellular diffusion of latent heat. The effect of cryo-protective agent on the cell suspension of bovine fetal aorta endothelial cell (BFAEC) was examined by the observation of latent heat during the repeated freezing processes.

Citation Download Citation

Junko Morikawa, Toshimasa Hashimoto, Kimiko Yamamoto, and Joji Ando "Two-dimensional thermal analysis for freezing of endothelial cells by high-speed microscopic IR focal plane arrays", Proc. SPIE 5697, Photons Plus Ultrasound: Imaging and Sensing 2005: The Sixth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, (25 April 2005); https://doi.org/10.1117/12.591527

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