25 April 2005 Two-dimensional thermal analysis for freezing of endothelial cells by high-speed microscopic IR focal plane arrays
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Abstract
Two-dimensional micro scale thermal analysis is proposed for the measurement of the latent heat released from the cultured vascular endothelial cells during freezing by use of high-speed infrared focal plane arrays. The thermo graphically observed latent heat spreading over the tissues and the inter- or intracellular thermal diffusion were detected with a spatial resolution of 7.5mm. The temperature distribution in time and space in each endothelial cell was influenced by both the self-release and intercellular diffusion of latent heat. The effect of cryo-protective agent on the cell suspension of bovine fetal aorta endothelial cell (BFAEC) was examined by the observation of latent heat during the repeated freezing processes.
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Junko Morikawa, Toshimasa Hashimoto, Kimiko Yamamoto, Joji Ando, "Two-dimensional thermal analysis for freezing of endothelial cells by high-speed microscopic IR focal plane arrays", Proc. SPIE 5697, Photons Plus Ultrasound: Imaging and Sensing 2005: The Sixth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, (25 April 2005); doi: 10.1117/12.591527; https://doi.org/10.1117/12.591527
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