A novel DNA restriction technology based on laser pulse energy conversion on sequence-specific bound metal nanoparticles

Andrea Csaki; Gunter Maubach; Frank Garwe; Andrea Steinbrueck; Karsten Koenig; Wolfgang Fritzsche

doi:10.1117/12.590450

29 March 2005 A novel DNA restriction technology based on laser pulse energy conversion on sequence-specific bound metal nanoparticles

Andrea Csaki, Gunter Maubach, Frank Garwe, Andrea Steinbrueck, Karsten Koenig, Wolfgang Fritzsche

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Proceedings Volume 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III; (2005) https://doi.org/10.1117/12.590450
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

DNA restriction is a basic method in today’s molecular biology. Besides application for DNA manipulation, this method is used in DNA analytics for 'restriction analysis'. Thereby DNA is digested by sequence specific restriction enzymes, and the length distribution of the resulting fragments is detected by gel electrophoresis. Differences in the sequence lead to different restriction patterns. A disadvantage of this standard method is the limitation to a small set of fixed sequences, so that the assay can not be adapted to any sequence of interest (e.g. SNP). We designed a scheme for DNA restriction in order to provide access to any desired sequence, based on laser light conversion on sequence-specific positioned metal nanoparticles. Especially gold nanoparticles are known for their interesting optical properties caused by plasmon resonance. The resulting absorption can be used to convert laser light pulses into heat, resulting in nanoparticle destruction. We work on the combination of this principle with DNA-modification of nanoparticles and the sequence-specific binding (hybridization) of these DNA-nanoparticle complexes along DNA molecules. Different mechanisms of light-conversion were studied, and the destructive effect of laser light on the nanoparticles and DNA is demonstrated.

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Andrea Csaki, Gunter Maubach, Frank Garwe, Andrea Steinbrueck, Karsten Koenig, and Wolfgang Fritzsche "A novel DNA restriction technology based on laser pulse energy conversion on sequence-specific bound metal nanoparticles", Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); https://doi.org/10.1117/12.590450

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KEYWORDS

Nanoparticles

Particles

Molecules

Metals

Gold

Atomic force microscopy

Laser applications

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