30 March 2005 Spectrally resolved fluorescence lifetime and FRET measurements
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Abstract
We present two different approaches that allow multi-wavelength fluorescence lifetime measurements in the time domain in conjunction with a laser scanning microscope and a pulsed excitation source. One technique is based on a streak camera system, the other technique is based on a time-correlated-single-photon-counting (TCSPC) approach. When applied to Forster resonance energy transfer (FRET) measurements, these setups are capable to record time-resolved fluorescence decays for the donor and the acceptor simultaneously.
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Christoph U. Biskup, Christoph U. Biskup, Laimonas Kelbauskas, Laimonas Kelbauskas, Thomas Zimmer, Thomas Zimmer, Sascha Dietrich, Sascha Dietrich, Klaus Benndorf, Klaus Benndorf, Wolfgang Becker, Wolfgang Becker, Axel Bergmann, Axel Bergmann, Nikolaj Klocker, Nikolaj Klocker, } "Spectrally resolved fluorescence lifetime and FRET measurements", Proc. SPIE 5700, Multiphoton Microscopy in the Biomedical Sciences V, (30 March 2005); doi: 10.1117/12.613006; https://doi.org/10.1117/12.613006
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