Microlymphatic flow using fast video microscopy

J. Brandon Dixon; David C. Zawieja; Anatoly A. Gashev M.D.; Gerard L. Cote

doi:10.1117/12.588633

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24 March 2005 Microlymphatic flow using fast video microscopy

J. Brandon Dixon, David C. Zawieja, Anatoly A. Gashev M.D., Gerard L. Cote

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Proceedings Volume 5701, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XII; (2005) https://doi.org/10.1117/12.588633
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Despite advances in the measurement of lymphatic flow, little is known about the actual velocities of flow in microlymphatic (~100 um diameter) vessels. In this paper, video microscopy and particle tracking methods were adapted and integrated with an ultra high-speed imaging camera to obtain measurements of high-speed lymph velocities that previous systems were incapable of measuring. In this study, a mesenteric microlymphatic vessel in a loop of the small intestine of a male Sprague-Dawley rat was exteriorized and imaged at a rate of 500 frames per second (fps) for several contraction sequences. Lymph velocity was shown to fluctuate cyclically with the vessel wall contractions and ranged from -1 to 4 mm/sec through a ten second sequence.

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J. Brandon Dixon, David C. Zawieja, Anatoly A. Gashev M.D., and Gerard L. Cote "Microlymphatic flow using fast video microscopy", Proc. SPIE 5701, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XII, (24 March 2005); https://doi.org/10.1117/12.588633

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