7 March 2005 NIR-integrating sphere spectroscopy to determine various blood properties
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Abstract
Near infrared integrating sphere spectroscopy and chemometric multivariate calibration were applied to determine hematocrit (HCT) and oxygen saturation (O2Sat) of circulating human blood. The diffuse reflectance were measured and the partial least square method (PLS) was used for calibration considering different wavelength ranges. The HCT and the O2Sat could be predicted with a root mean square error (PRMSE) of 1.9% and 2.8% respectively, using PLS. Each parameter was adjusted to various levels, and three measurement series from blood of three different donors were carried out for the calibration with the PLS. The calibration includes changes in hemolysis and osmolarity as well as inter-individual differences in cell dimensions and hemoglobin content. Prediction of hemolysis was also possible for one blood sample with a PRMSE of 0.8%.
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Martina Meinke, Martina Meinke, Moritz Friebel, Moritz Friebel, Ingo H. Gersonde, Ingo H. Gersonde, Gerhard J. Muller, Gerhard J. Muller, } "NIR-integrating sphere spectroscopy to determine various blood properties", Proc. SPIE 5702, Optical Diagnostics and Sensing V, (7 March 2005); doi: 10.1117/12.588495; https://doi.org/10.1117/12.588495
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