Chemical luminescence measurement of singlet oxygen generated by photodynamic therapy in solutions in real time

Shiming Luo; Da Xing; Jing Zhou; Yanfang Qin; Qun Chen

doi:10.1117/12.589497

4 April 2005 Chemical luminescence measurement of singlet oxygen generated by photodynamic therapy in solutions in real time

Shiming Luo, Da Xing, Jing Zhou, Yanfang Qin, Qun Chen

Author Affiliations +

Proceedings Volume 5704, Genetically Engineered and Optical Probes for Biomedical Applications III; (2005) https://doi.org/10.1117/12.589497
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Photodynamic therapy (PDT) is a cancer therapy that utilizes optical energy to activate a photosensitizer drug in a target tissue. Reactive oxygen species (ROS), such as ¹O₂ and superoxide, are believed to be the major cytotoxic agents involved in PDT. Although current PDT dosimetry mostly involves measurements of light and photosensitizer doses delivered to a patient, the quantification of ROS production during a treatment would be the ultimate dosimetry of PDT. Technically, it is very difficult and expensive to directly measure the fluorescence from ¹O₂, due to its extreme short lifetime and weak signal strength. In this paper, Photofrin(R) and 635nm laser were used to generate ¹O₂ and superoxide in a PDT in solution. Compound 3,7- dihydro-6-{4-[2-(N’-(5-fluoresceinyl) thioureido) ethoxy] phenyl}-2- methylimidazo{1,2-a} pyrazin-3-one sodium salt,an Cyp- ridina luciferin analog commonly referred as FCLA, was used as a chemical reporter of ROS. The 532nm chemiluminescence (CL) from the reaction of the FCLA and ROS was detected with a photon multiplier tube (PMT) system operating at single photon counting mode. With the setup, we have made detections of ROS generated by PDT in real time. By varying the amount of conventional PDT dosage (photosensitizer concentration, light irradiation fluence and its delivery rate) and the amount of FCLA, the intensity of CL and its consumption rate were investigated. The results show that the intensity and temporal profile of CL are highly related to the PDT treatment parameters. This suggests that FCLA CL may provide a highly potential alternative for ROS detection during PDT.

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Shiming Luo, Da Xing, Jing Zhou, Yanfang Qin, and Qun Chen "Chemical luminescence measurement of singlet oxygen generated by photodynamic therapy in solutions in real time", Proc. SPIE 5704, Genetically Engineered and Optical Probes for Biomedical Applications III, (4 April 2005); https://doi.org/10.1117/12.589497

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KEYWORDS

Photodynamic therapy

Oxygen

Luminescence

Chemiluminescence

Single photon

Photosensitizer targeting

Analog electronics

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