4 April 2005 Luminescent probes for optical in vivo imaging
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Abstract
Going along with instrumental development for small animal fluorescence in vivo imaging, we are developing molecular fluorescent probes, especially for tumor targeting. Several criteria have to be taken into account for the optimization of the luminescent label. It should be adapted to the in vivo imaging optical conditions : red-shifted absorption and emission, limited overlap between absorption and emission for a good signal filtering, optimized luminescence quantum yield, limited photo-bleaching. Moreover, the whole probe should fulfill the biological requirements for in vivo labeling : adapted blood-time circulation, biological conditions compatibility, low toxicity. We here demonstrate the ability of the imaging fluorescence set-up developed in LETI to image the bio-distribution of molecular probes on short times after injection. Targeting with Cy5 labeled holo-transferrin of subcutaneous TS/Apc (angiogenic murine breast carcinoma model) or IGROV1 (human ovarian cancer) tumors was achieved. Differences in the kinetics of the protein uptake by the tumors were evidenced. IGROV1 internal metastatic nodes implanted in the peritoneal cavity could be detected in nude mice. However, targeted metastatic nodes in lung cancer could only be imaged after dissection of the mouse. These results validate our fluorescence imaging set-up and the use of Cy5 as a luminescent label. New fluorescent probes based on this dye and a molecular delivery template (the RAFT molecule) can thus be envisioned.
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Isabelle Texier, Isabelle Texier, Veronique Josserand, Veronique Josserand, Elisabeth Garanger, Elisabeth Garanger, Jesus Razkin, Jesus Razkin, Zhaohui Jin, Zhaohui Jin, Pascal Dumy, Pascal Dumy, Marie Favrot, Marie Favrot, Didier Boturyn, Didier Boturyn, Jean-Luc Coll, Jean-Luc Coll, } "Luminescent probes for optical in vivo imaging", Proc. SPIE 5704, Genetically Engineered and Optical Probes for Biomedical Applications III, (4 April 2005); doi: 10.1117/12.588162; https://doi.org/10.1117/12.588162
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