Fluorescent labeling of cells and biomolecules with nanocrystal quantum dots

Akiyoshi Hoshino; Kouki Fujioka; Masakazu Suga; Yu F. Sasaki; Toshihiro Ohta; Masato Yasuhara; Taeko Dohi; Kazuo Suzuki; Kenji Yamamoto

doi:10.1117/12.601614

28 April 2005 Fluorescent labeling of cells and biomolecules with nanocrystal quantum dots

Akiyoshi Hoshino, Kouki Fujioka, Masakazu Suga, Yu F. Sasaki, Toshihiro Ohta, Masato Yasuhara, Taeko Dohi, Kazuo Suzuki, Kenji Yamamoto

Author Affiliations +

Proceedings Volume 5705, Nanobiophotonics and Biomedical Applications II; (2005) https://doi.org/10.1117/12.601614
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Fluorescent nanoparticles, such as nanocrystal quantum dots (QDs), novel nanometer-size probes and have the potential to be used as easy imaging tool for molecular biology and bioimaging including medical applications, since some nanocrystals emit higher and far longer fluorescence than conventional organic probes. QDs are now becoming widely used in biotechnology and medical applications. QDs have several advantages over organic fluorophores with regard to high luminescence, stability against photobleaching, and a range of fluorescence wavelengths from blue to infrared depending on the particle size. In this review, we reported labeling of some kinds of immune cells and biomolecules with several QDs coated with hydrophilic carboxyl/amine groups, and reported that we could image the circulation of mouse lymphocytes in vivo by QDs. In addition, we also reported here about the cytotoxicity of these nanocrystals.

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Akiyoshi Hoshino, Kouki Fujioka, Masakazu Suga, Yu F. Sasaki, Toshihiro Ohta, Masato Yasuhara, Taeko Dohi, Kazuo Suzuki, and Kenji Yamamoto "Fluorescent labeling of cells and biomolecules with nanocrystal quantum dots", Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); https://doi.org/10.1117/12.601614

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