The quantum dot nanoconjugate tool box

I. D. Tomlinson; D. W. Wright; T. D. Giorgio; R. D. Blakely; S. J. Pennycook; D. Hercules; L. Bentzen; R. A. Smith; J. McBride; M. J. Vergne; S. Rosenthal

doi:10.1117/12.606592

28 April 2005 The quantum dot nanoconjugate tool box (Invited Paper)

I. D. Tomlinson, D. W. Wright, T. D. Giorgio, R. D. Blakely, S. J. Pennycook, D. Hercules, L. Bentzen, R. A. Smith, J. McBride, M. J. Vergne, S. Rosenthal

Author Affiliations +

Proceedings Volume 5705, Nanobiophotonics and Biomedical Applications II; (2005) https://doi.org/10.1117/12.606592
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

The surface coating of quantum dots has been characterised using Z-stem. Quantum dots have been pegylated to increase stability in aqueous solution. The fluorescence intensity of the quantum dots was modulated pegylation. PEG was coupled using different ratios of EDC, PEG and NHS. Optimum coupling conditions were found to occur when 2000 equivalents of PEG were reacted with 1 equivalent of dot in the presence of 1500 equivalents of NHS and EDC. Angiotensin II was also conjugated to quantum dots and these conjugates were shown to be biologically active. Quantum dots have also been surface functionalised with other peptides such as NGR with subsequent demonstration of cell surface binding and can be characterized by flow cytometry.

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I. D. Tomlinson, D. W. Wright, T. D. Giorgio, R. D. Blakely, S. J. Pennycook, D. Hercules, L. Bentzen, R. A. Smith, J. McBride, M. J. Vergne, and S. Rosenthal "The quantum dot nanoconjugate tool box (Invited Paper)", Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); https://doi.org/10.1117/12.606592

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