28 April 2005 Time-resolved fluorescence measurements using self-pulsing 650-nm laser diodes
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We report on the development of a stroboscopic excitation technique using a self-pulsing laser, and show that it is a feasible method for obtaining fluorescence lifetime information from a biochip format. The self-pulsing lasers described here are versatile devices which have been used for one photon excitation measurements to determine the lifetime of cyanine 5 in water and ethanol. The same devices have been used to develop a technique whereby the emphasis for time-resolution of a lifetime measurement can be transferred to the excitation source from the detector and processing electronics by virtue of the multiple-pulse, variable frequency nature of the laser output.
© (2005) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Daniel R. Matthews, Daniel R. Matthews, Huw D. Summers, Huw D. Summers, Kerenza Njoh, Kerenza Njoh, Rachel J. Errington, Rachel J. Errington, Paul J. Smith, Paul J. Smith, Simon Ameer-Beg, Simon Ameer-Beg, Boris Vojnovic, Boris Vojnovic, "Time-resolved fluorescence measurements using self-pulsing 650-nm laser diodes", Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); doi: 10.1117/12.590201; https://doi.org/10.1117/12.590201

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