21 March 2005 Nerve regeneration after femtosecond laser nanosurgery
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We demonstrate sub-micron scale surgery with femtosecond lasers in a tiny living organism. By just cutting few nano-scale nerve connections inside the nematode C. elegans, we succeeded to stop the whole animal from moving backwards. This delicate axotomy keeps the surrounding of the severed axons un-damaged so that the axons can regrow back, and the worms recover and can move backwards again. These results demonstrate, for the first time, nerve regeneration in such a tiny organism, in its evolutionarily simplest form. The ability to perform precise sub-micron scale axotomy on such organisms provides tremendous research potential for rapid screening of drugs and discovery of new biomolecules affecting regeneration and development.
© (2005) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Mehmet Fatih Yanik, Mehmet Fatih Yanik, Hulusi Cinar, Hulusi Cinar, Hediye Nese Cinar, Hediye Nese Cinar, Andrew D. Chisholm, Andrew D. Chisholm, Yishi Jin, Yishi Jin, Adela Ben-Yakar, Adela Ben-Yakar, "Nerve regeneration after femtosecond laser nanosurgery", Proc. SPIE 5714, Commercial and Biomedical Applications of Ultrafast Lasers V, (21 March 2005); doi: 10.1117/12.613174; https://doi.org/10.1117/12.613174

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