22 January 2005 Adsorption of fluorescently labeled microbeads on PDMS surfaces
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Abstract
Fluorescently labeled beads may be utilized in transparent microfluidic devices to facilitate a variety of immunoassay based chemical measurements. We investigate the ability to visualize, quantitate, and reduce undesirable adsorption of beads within a polydimethylsiloxane (PDMS) microchannel device. These methods are prerequisites to the design of practical bead-based microfluidic sensing devices. The PDMS microchannels were shown to be transparent enough to make accurate quantitative optical measurements, although significant adsorption was observed. Epifluorescence microscopy was employed in an attempt to quantitatively evaluate microbead adsorption to PDMS microchannel walls and bulk surfaces after different agitation, solution, and surface treatments. This microscopy method provides reproducible imaging of individual beads and allows for characterization of adsorption to PDMS microchannel walls. Solution composition seemed to play a more important role in the ability to reduce the number of adsorbed beads to the PDMS surface than agitation. The most significant reduction in bead adsorption was seen in surface treatment. The most effective surface treatment examined in this study was Teflon AF.
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Irena Nikcevic, Adam Bange, Erik T. K. Peterson, Ian Papautsky, William R. Heineman, H. Brian Halsall, Carl J. Seliskar, "Adsorption of fluorescently labeled microbeads on PDMS surfaces", Proc. SPIE 5718, Microfluidics, BioMEMS, and Medical Microsystems III, (22 January 2005); doi: 10.1117/12.591781; https://doi.org/10.1117/12.591781
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