22 January 2005 Fluorescence calibration research of microspheres in a microfluidic chip
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Fluorescence calibration is usually done by preparing bio-samples with a series of concentrations and measuring their corresponding fluorescence intensities. A simplified approach is studied by using a microfluidic chip and microspheres. The fluorescence calibration can be carried out on the chip with only one concentration of the microspheres. Microspheres with the diameter of 1~5μm are very useful in bio-detection research. These microspheres are manufactured using high-quality, ultraclean polystyrene microspheres and loaded with a variety of proprietary dyes. They can be labeled with biotin-, NeutrAvdin-, streptavidin- and protein, which can be used as tracers for bio-detections. A microfluidic chip was successfully fabricated for the experiment, and preliminary experimental results have proved the feasibility of the approach for fluorescence calibration.
© (2005) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Lin Wang, Lin Wang, Kun Lian, Kun Lian, Jost Goettert, Jost Goettert, "Fluorescence calibration research of microspheres in a microfluidic chip", Proc. SPIE 5718, Microfluidics, BioMEMS, and Medical Microsystems III, (22 January 2005); doi: 10.1117/12.591610; https://doi.org/10.1117/12.591610

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