22 January 2005 Fluorescence calibration research of microspheres in a microfluidic chip
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Abstract
Fluorescence calibration is usually done by preparing bio-samples with a series of concentrations and measuring their corresponding fluorescence intensities. A simplified approach is studied by using a microfluidic chip and microspheres. The fluorescence calibration can be carried out on the chip with only one concentration of the microspheres. Microspheres with the diameter of 1~5μm are very useful in bio-detection research. These microspheres are manufactured using high-quality, ultraclean polystyrene microspheres and loaded with a variety of proprietary dyes. They can be labeled with biotin-, NeutrAvdin-, streptavidin- and protein, which can be used as tracers for bio-detections. A microfluidic chip was successfully fabricated for the experiment, and preliminary experimental results have proved the feasibility of the approach for fluorescence calibration.
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Lin Wang, Lin Wang, Kun Lian, Kun Lian, Jost Goettert, Jost Goettert, "Fluorescence calibration research of microspheres in a microfluidic chip", Proc. SPIE 5718, Microfluidics, BioMEMS, and Medical Microsystems III, (22 January 2005); doi: 10.1117/12.591610; https://doi.org/10.1117/12.591610
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