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22 June 2005 Optics in cell and tissue engineering (Invited Paper)
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Optical methods continue to play significant roles in medical and biological research. In recent times cell and tissue engineering have emerged rapidly and are now well recognised as important multi-disciplinary endeavours within the medical and biological spheres. This paper describes applications of optical science and technology in cell and tissue engineering, ranging from the basic visualization of organeeles, cells and tissues through to probing the structure and function of cells and tissues being cultured in bioreactors. The development of tissue engineering for the fabrication of cell-based systems, for example for the repair or replacement of damaged or diseased tissues and organs, is first described. Then, interactions of light propagating through cells and tissues are considered, both theoretically and practically, as a prelude to the description of means for optical interrogation. Scattering and absorption of light by cells and tissues is shown to provide useful opportunities for measurement, for example via spectrophotometric analysis or small angle light scattering. Optical sensors for chemical analysis based on immobilised fluorophores and chromophores are also presented, these being particularly useful in bioreactors. In this application the continuous monitoring of a variety of chemical and physical variables may lead to optimised cell and tissue culture.
© (2005) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
P. Rolfe "Optics in cell and tissue engineering (Invited Paper)", Proc. SPIE 5771, Saratov Fall Meeting 2004: Optical Technologies in Biophysics and Medicine VI, (22 June 2005);

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