3 June 2005 A fluorescence methodology for assessing the polarity and composition of novel thermoresponsive hydrophylic/hydrophobic copolymer system (Invited Paper)
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Abstract
The use of designed polymer coatings for specific applications such as drug delivery or modifying cell response is a critical aspect of medical device manufacturing. The chemical composition and physical characteristics of thin polymer coatings need to be analysed in-situ and this can present difficulties for traditional analytical methods. For example, changes in the polarity of polymer coatings are typically measured using the contact angle (CA) method. This is a simple process and gives good results however; it cannot be used to measure very hydrophilic polymers, or to analyse features smaller than a couple of mm in size. There is a need for a non-contact method for polarity measurement that is suitable for hydrophilic polymers on a macro- and microscopic scale. 4'-diethylamino-3-hydroxyflavone (FE), 5, 6-benzo-4'-diethylamino-3-hydroxyflavone (BFE), and 4'-diethylamino-3-hydroxy-7-methoxyflavone (MFE) are fluorescence probes based on 3-hydroxyflavone. They respond to environment perturbations by shift and changes in the relative intensity of two well-separated bands in the emission spectra. These bands originate from an excited state intramolecular proton transfer (ESIPT) reaction. We have incorporated FE, BFE, and MFE into a novel thermoresponsive hydrophilic/hydrophobic copolymer system (NIPAM-NtBA) and studied its fluorescence behaviour. The fluorescence emission spectra depend strongly on copolymer composition, with increasing hydrophobicity (greater NtBA fraction) leading to a decrease in the value of log (IN*/IT*). This allows for the non-contact, measurement of the exact composition and surface energy of the copolymer system.
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Boguslaw Szczupak, Boguslaw Szczupak, Alan G. Ryder, Alan G. Ryder, Yuri A. Rochev, Yuri A. Rochev, Andrey S. Klymchenko, Andrey S. Klymchenko, Alexander Gorelov, Alexander Gorelov, Thomas J. Glynn, Thomas J. Glynn, } "A fluorescence methodology for assessing the polarity and composition of novel thermoresponsive hydrophylic/hydrophobic copolymer system (Invited Paper)", Proc. SPIE 5826, Opto-Ireland 2005: Optical Sensing and Spectroscopy, (3 June 2005); doi: 10.1117/12.605117; https://doi.org/10.1117/12.605117
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