Fluorescence imaging spectroscopy utilising acousto-optic tuneable filters

Mounir Bouhifd; Maurice P. Whelan; Marc Aprahamian

doi:10.1117/12.605088

3 June 2005 Fluorescence imaging spectroscopy utilising acousto-optic tuneable filters

Mounir Bouhifd, Maurice P. Whelan, Marc Aprahamian

Proceedings Volume 5826, Opto-Ireland 2005: Optical Sensing and Spectroscopy; (2005) https://doi.org/10.1117/12.605088
Event: OPTO-Ireland, 2005, Dublin, Ireland

Abstract

A novel prototype instrument for mutli-spectral imaging applications is described. The device is based on an acousto-optic tuneable filter (AOTF) that can be easily attached to many standard imaging systems (e.g. endoscope or fluorescence microscope). The instrument developed offers significant advantages over typical fixed-filter based systems in terms of flexibility, performance and diagnostic potential. The selected AOTF was designed to have a large acceptance aperture suitable for imaging applications. Any filtering centre-wavelength in the visible range (450 to 700nm) can be rapidly selected by either random access or by continuous tuning thus providing a versatile performance. The prototype instrument has been demonstrated for in-vivo applications where it was attached to the eyepiece of a commercial endoscope allowing simultaneous white light and fluorescence endoscopy. Autofluorescence of endogenous protoporphyrin IX (PpIX), a biomarker of diseased tissues undergoing an inflammatory response, was mapped in vivo on a rat model. The AOTF device was also coupled to the viewing port of a commercial fluorescence microscope thus realising a powerful fluorescence imaging spectrometer capable of detecting and mapping fluorescent biomolecules in vitro.

Citation Download Citation

Mounir Bouhifd, Maurice P. Whelan, and Marc Aprahamian "Fluorescence imaging spectroscopy utilising acousto-optic tuneable filters", Proc. SPIE 5826, Opto-Ireland 2005: Optical Sensing and Spectroscopy, (3 June 2005); https://doi.org/10.1117/12.605088

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