22 April 2005 In vitro cytotoxicity assessment of phthalocyanines on virus-transformed animal cells
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Proceedings Volume 5830, 13th International School on Quantum Electronics: Laser Physics and Applications; (2005) https://doi.org/10.1117/12.618977
Event: 13th International School on Quantum Electronics: Laser Physics and Applications, 2004, Bourgas, Bulgaria
Abstract
In this study we evaluate in vitro the cytotoxic activity of two newly synthesized phthalocyanines (metallo-complexes) with absorption bands in the red part of the spectrum: zinc-tri-sulphonated phthalocyanine (ZnS4Pc). Two permanent animal tumor cell lines are used in the experiments: LSR-SF(SR)-transplantable sarcoma in rat induced by Rous sarcoma virus strain Schmidt-Ruppin and LSCC-SF(Mc29)-transplantable chicken hepatoma induced by the myelocytomatosis virus Mc29. Irradiation has been performed with a laser diode emitting at 672 nm in a wide range of radiant exposure (2-100 J/cm2) and irradiance of 120 mW/cm2. The neutral red uptake cytotoxicity assay has been used to evaluate the effect of the photosensitizers on cell viability. The light dose-response curves and light exposures that ensure viability drop to 50% and 10% have been obtained for each cell line. The ability of the compounds to induce apoptosis has been determined by acridine orange dye staining.
© (2005) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Radostina Alexandrova, Radostina Alexandrova, Elena Stoykova, Elena Stoykova, Rodica-Mariana Ion, Rodica-Mariana Ion, Kristina Nedkova, Kristina Nedkova, Elena Ivanova, Elena Ivanova, Kaloian Zdravkov, Kaloian Zdravkov, Georgi Minchev, Georgi Minchev, } "In vitro cytotoxicity assessment of phthalocyanines on virus-transformed animal cells", Proc. SPIE 5830, 13th International School on Quantum Electronics: Laser Physics and Applications, (22 April 2005); doi: 10.1117/12.618977; https://doi.org/10.1117/12.618977
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