7 October 2005 Fluorescence spectroscopy in tissue phantoms for improved depth resolution in tissue imaging
Author Affiliations +
A way to determine the depth of an embedded fluorescent object, for example deep-lying tumors marked with a fluorescent probe, is to detect fluorescent light that has propagated through the medium at two different wavelength bands. A ratio can then be calculated between the corresponding intensities. The wavelength regions should be chosen such that there is a difference in the absorption in the medium. This spectral information could be used as a complement in other methods, for example in tomography, due to its straightforward implementation. In this study we have performed phantom measurements to determine the depth of a fluorescent object, filled with fluorophores. The transmission of yellow and red fluorescence was measured and a ratio of yellow to red fluorescence was calculated for several depths in tissue with a thickness of 2 cm. The ratio showed a clear dependence on the depth of the object.
© (2005) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jenny Svensson, Jenny Svensson, Anikitos Garofalakis, Anikitos Garofalakis, Heiko Meyer, Heiko Meyer, Florian Forster, Florian Forster, Jorge Ripoll, Jorge Ripoll, Stefan Andersson-Engels, Stefan Andersson-Engels, } "Fluorescence spectroscopy in tissue phantoms for improved depth resolution in tissue imaging", Proc. SPIE 5859, Photon Migration and Diffuse-Light Imaging II, 585906 (7 October 2005); doi: 10.1117/12.632826; https://doi.org/10.1117/12.632826

Back to Top