Interferometric tracking of two particles with dynamic optical traps

Dirk Neumayer; Peter Kaiser; Ernst H.K. Stelzer; Alexander Rohrbach

doi:10.1117/12.632831

7 October 2005 Interferometric tracking of two particles with dynamic optical traps

Dirk Neumayer, Peter Kaiser, Ernst H.K. Stelzer, Alexander Rohrbach

Author Affiliations +

Proceedings Volume 5859, Photon Migration and Diffuse-Light Imaging II; 58590A (2005) https://doi.org/10.1117/12.632831
Event: European Conferences on Biomedical Optics 2005, 2005, Munich, Germany

Abstract

Optical potential landscapes for diffusing particles can be generated by time-multiplexing (scanning) an optical trap and additionally modulating the trapping laser power. We show that it is possible to determine the lateral and axial positions of several particles in parallel with some 10 nm precision and at kHz rates by using dynamic back focal plane interferometry. This allows measuring the interaction of diffusing, (non-) functionalized particles in a confined volume. The scan frequency of the optical trap can be optimized for interaction measurements with high dynamic or temporal resolution, given a data sampling frequency of 1MHz.

Citation Download Citation

Dirk Neumayer, Dirk Neumayer, Peter Kaiser, Peter Kaiser, Ernst H.K. Stelzer, Ernst H.K. Stelzer, Alexander Rohrbach, Alexander Rohrbach, } "Interferometric tracking of two particles with dynamic optical traps", Proc. SPIE 5859, Photon Migration and Diffuse-Light Imaging II, 58590A (7 October 2005); doi: 10.1117/12.632831; https://doi.org/10.1117/12.632831

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