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7 October 2005 Frequency-domain optical diffusion tomography of fluorescent proteins
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Abstract
We present preliminary results of the frequency domain fluorescent diffuse tomography (FD FDT) method in application to fluorescent proteins. For first step in the experimental setup we utilized light-emitting diode (530 nm wavelength) modulated with low frequency (18 kHz). A model experiments with capsules containing DsRed suspension in scattering medium has been conducted. The results of post mortem experiments with capsules containing DsRed, introduced into abdominal cavity of mice to simulate tumors inside animal body, are presented. An algorithm of processing fluorescent image based on calculating zero of maximum curvature has been applied to detect fluorescent inclusions boundaries on the image.
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Ilya V. Turchin, Vladimir I. Plehanov, Ekaterina A. Sergeeva, Anna G. Orlova, Vladimir A. Vorob'ev, Vladislav A. Kamensky, and Alexander P. Savitsky "Frequency-domain optical diffusion tomography of fluorescent proteins", Proc. SPIE 5859, Photon Migration and Diffuse-Light Imaging II, 58591H (7 October 2005); doi: 10.1117/12.632879; https://doi.org/10.1117/12.632879
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