8 October 2005 Observation of collagen fiber structure in dermis tissue by a second-harmonic-generation microscope
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Second-harmonic-generation (SHG) microscope is applied to observe collagen fiber structure in porcine dermis and mouse tendon. Difference of collagen fiber structure among different samples is clearly visualized as high contrast SHG images using a sample-scanning SHG microscope based on a transmission configuration. From comparison of the SHG image between a transmission detection mode and a reflection one, we confirm that the reflection-mode SHG imaging is also useful to observe the collagen fiber structure in the porcine dermis. Finally, we applied a laser-scanning confocal SHG microscope to optical-sectioning SHG imaging of the thick porcine dermis every 10-μm depth and confirmed that the collagen fiber structure in the dermis is spatially evolved along the depth direction. The proposed method will be a powerful tool for in vivo measurement of human dermis to monitor skin diseases and cosmetics.
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T. Yasui, M. Ito, K. Sasaki, T. Araki, T. Yamashita, N. Kunizawa, M. Takahashi, "Observation of collagen fiber structure in dermis tissue by a second-harmonic-generation microscope", Proc. SPIE 5860, Confocal, Multiphoton, and Nonlinear Microscopic Imaging II, 58600O (8 October 2005); doi: 10.1117/12.632959; https://doi.org/10.1117/12.632959

Second-harmonic generation


Structured optical fibers



Tissue optics


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