26 August 2005 Femtosecond laser manipulation of subcellular organelles in living cells
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Abstract
Manipulation—that is, inactivation and movement—of subcellular organelles in living cells is important to understand cell dynamics. We review disruption of subcellular organelles in living cells by tightly focusing femtosecond laser pulses inside the cells. Photodisruption of organelles in living cells was experimentally confirmed by restaining of organelles. Femtosecond laser based nanosurgery has the possibility to remove or manipulate organelles in living cells and may lead to elucidation of functional interactions between organelles, protein transportation, and inter-cell communication.
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Wataru Watanabe, Wataru Watanabe, Tomoko Shimada, Tomoko Shimada, Kazuyoshi Itoh, Kazuyoshi Itoh, Sachihiro Matsunaga, Sachihiro Matsunaga, Kiichi Fukui, Kiichi Fukui, } "Femtosecond laser manipulation of subcellular organelles in living cells", Proc. SPIE 5863, Therapeutic Laser Applications and Laser-Tissue Interactions II, 58630B (26 August 2005); doi: 10.1117/12.633079; https://doi.org/10.1117/12.633079
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