1 September 2005 Time-resolved fluorescence measurements using stroboscopic excitation
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We report on the development of a simple technique for obtaining time-domain information using dc detection of fluorescence. We show that this is feasible for assays where a change in lifetime of an indicator occurs in reaction to an analyte, in fluorescence resonance energy transfer for example, and could be particularly useful for assays performed in the scaled-down environment of a "lab-on-a-chip". A rate equation model is presented which allows an objective analysis of the relative importance of the key measurement parameters: optical saturation of the fluorophore and excitation pulse characteristics. We present a comparison of the model with a cuvette based analysis of a carbocyanine dye where the excitation source is a 650 nm wavelength, self-pulsing AlGaInP laser diode.
© (2005) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Daniel R. Matthews, Daniel R. Matthews, Huw D. Summers, Huw D. Summers, Kerenza Njoh, Kerenza Njoh, Rachel J. Errington, Rachel J. Errington, Paul J. Smith, Paul J. Smith, Simon Ameer-Beg, Simon Ameer-Beg, Boris Vojnovic, Boris Vojnovic, "Time-resolved fluorescence measurements using stroboscopic excitation", Proc. SPIE 5864, Novel Optical Instrumentation for Biomedical Applications II, 586409 (1 September 2005); doi: 10.1117/12.632914; https://doi.org/10.1117/12.632914

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