Design and development of multifunctional confocal laser scanning microscope with UV/VIS laser source

Yoshikazu Kanai; Yousuke Kanzaki; Moriaki Wakaki; Norihide Takeyama

doi:10.1117/12.616637

23 August 2005 Design and development of multi functional confocal laser scanning microscope with UV / VIS laser source

Yoshikazu Kanai, Yousuke Kanzaki, Moriaki Wakaki, Norihide Takeyama

Proceedings Volume 5878, Advanced Characterization Techniques for Optics, Semiconductors, and Nanotechnologies II; 58781D (2005) https://doi.org/10.1117/12.616637
Event: Optics and Photonics 2005, 2005, San Diego, California, United States

Abstract

A high resolution Confocal Laser Scanning Microscope (CLSM) with UV / VIS light sources was developed as the first step of multi-functional microscope. The optical system is designed to optimize for both UV and VIS wavelengths. An UV laser is used to achieve higher resolution, and a VIS is for multi functions. A new objective lens specialized for this application was designed and fabricated. Specification of the lens and the optical system is NA:0.95, EFL:2.5mm, WD:1.5mm, Resolution:160nm and achromatic for two wavelengths of UV 325.0nm / VIS 632.8nm. Several specimens were characterized to check the performance of the system. Some optical materials under study were measured for evaluation, and interesting results could be obtained. Multi-functional measurements are being planed as a next step. This system will help the research of nano-structures, photonic-crystals and biology.

Citation Download Citation

Yoshikazu Kanai, Yousuke Kanzaki, Moriaki Wakaki, and Norihide Takeyama "Design and development of multi functional confocal laser scanning microscope with UV / VIS laser source", Proc. SPIE 5878, Advanced Characterization Techniques for Optics, Semiconductors, and Nanotechnologies II, 58781D (23 August 2005); https://doi.org/10.1117/12.616637

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