26 August 2005 Characterization of SERS substrates for chem/bio processing systems
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Proceedings Volume 5927, Plasmonics: Metallic Nanostructures and Their Optical Properties III; 59270P (2005); doi: 10.1117/12.617871
Event: Optics and Photonics 2005, 2005, San Diego, California, United States
Surface enhanced Raman spectroscopy (SERS) has promise as an optical sensor for the detection of chemical and biological agents, in particular when combined with front-end processing for sample preparation prior to analysis. In this paper, we report preliminary results from a SERS analysis of Bacillus cereus T strain (BcT), which was prepared for sensor analysis via a microfluidics-based sample processor. In the microfluidics hardware, low and high molecular weight analytes from a sonicated spore sample were separated via mass-dependent diffusion into two independent microchannels. SERS analysis of the sample outputs revealed a significant separation of the low molecular spore biomarker, dipicolinic acid, from the high molecular weight protein and nucleic acid background. In addition to the processing study, measurements were performed on gold core-shell nanospheres, which are considered a potential SERS substrate for the microfluidic system. Finally, field-induced aggregation of silver nanoparticles, an alternative to chemical aggregation, was shown to be an effective approach for the production of highly enhancing SERS substrates.
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Joseph A. Miragliotta, Jennifer L. Sample, Stergios J. Papadakis, "Characterization of SERS substrates for chem/bio processing systems", Proc. SPIE 5927, Plasmonics: Metallic Nanostructures and Their Optical Properties III, 59270P (26 August 2005); doi: 10.1117/12.617871; https://doi.org/10.1117/12.617871

Surface enhanced Raman spectroscopy




Statistical analysis

Raman spectroscopy

Chemical analysis

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