26 August 2005 Surface-enhanced Raman scattering (SERS) spectroscopy technique for lactic acid in serum measurement
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Highly sensitive measurement of biomolecules is very important in clinical diagnosis and biomedical sensing. Spectroscopic methods have played important roles in biomedical sensing system developments. Recent development in surface enhanced Raman scattering (SERS) method has greatly enhanced the weak Raman signals of biomolecules and has provided great potentials for real time measurement of biomolecules of body fluid. In addition, Raman measurement has the advantage of not requiring extrinsic fluorescent marker for labeling purpose. In this study, we have pioneered in the development of SERS spectroscopic measurement technique for serum lactic acid, which is one of the most important metabolic parameter in blood. We have fabricated Ag colloidal nanoparticles to enhance the weak Raman signal of lactic acid in serum. The diameter of the Ag nanoparticle is 20 nm, the nanoparticles concentration is 109particles/ml. We have observed the SERS characteristic peak of lactic acid at 1285~1480cm-1 under 632.8 nm HeNe laser excitation. We have demonstrated the measurement of the lactic acid in filtered serum in the physiological concentration range 5x10-3~22x10-3 mole/L, which is hundred times lower than the detectible range using traditional Raman approach. The serum samples with were measured in a specially designed reflector type sample holder to form a multiple reflection of excitation laser through the sample, between a reflector and a notch filter. In conclusion, this research demonstrates the feasibility of using Ag SERS technique for measuring the lactic acid at physical concentration and establishes the platform technique for human body fluid measurements.
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Hui Hua Kenny Chiang, Hui Hua Kenny Chiang, Po Hsiang Hsu, Po Hsiang Hsu, } "Surface-enhanced Raman scattering (SERS) spectroscopy technique for lactic acid in serum measurement", Proc. SPIE 5927, Plasmonics: Metallic Nanostructures and Their Optical Properties III, 59271Z (26 August 2005); doi: 10.1117/12.618127; https://doi.org/10.1117/12.618127

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