Analysis of the swimming activity of Pseudomonas aeruginosa by using photonic force microscope

Chia-Han Chan; Bo-Jui Chang; Ying-Jung Huang; Chia-Chieh Fan; Hwei-Ling Peng; Sien Chi; Long Hsu

doi:10.1117/12.616704

18 August 2005 Analysis of the swimming activity of Pseudomonas aeruginosa by using photonic force microscope

Chia-Han Chan, Bo-Jui Chang, Ying-Jung Huang, Chia-Chieh Fan, Hwei-Ling Peng, Sien Chi, Long Hsu

Proceedings Volume 5930, Optical Trapping and Optical Micromanipulation II; 59300E (2005) https://doi.org/10.1117/12.616704
Event: Optics and Photonics 2005, 2005, San Diego, California, United States

Abstract

Swimming activity of flagella is a main factor of the motility of bacteria. Flagella expressed on the surface of bacterial species serve as a primary means of motility including swimming. We propose to use optical tweezers to analyze the swimming activity of bacteria. The sample bacteria in the work is Pseudomonas aeruginosa, and it is a gram-negative bacterium and often causes leading to burn wound infections, urinary-tract infections, and pneumonia. The single polar flagellum of P. aeruginosa has been demonstrated to be important virulence and colonization factor of this opportunistic pathogen. We demonstrate a gene to regulate the bacterial swimming activity in P. aeruginosa PAO1 by biological method. However, the change of flagellar morphology was not observed by electron microscopy analysis, suggesting that the gene regulates the flagellar rotation that could not be detected by biological method. PFM exhibits a spatial resolution of a few nanometers to detect the relative position of the probe at an acquisition rate over 1 MHz. By binding a probe such as a bead or a quantum dot on the flagella, we expect the rotation of the probe due to the flagella could be detected. It is expected that the study of the swimming activity of P. aeruginosa provide potent method for the pathogenic role of the flagella in P. aeruginosa.

Citation Download Citation

Chia-Han Chan, Bo-Jui Chang, Ying-Jung Huang, Chia-Chieh Fan, Hwei-Ling Peng, Sien Chi, and Long Hsu "Analysis of the swimming activity of Pseudomonas aeruginosa by using photonic force microscope", Proc. SPIE 5930, Optical Trapping and Optical Micromanipulation II, 59300E (18 August 2005); https://doi.org/10.1117/12.616704

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