18 August 2005 Molecular binding and enhanced photoluminescence of bromocresol purple in marine derived DNA
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Abstract
Deoxyribonucleic acid (DNA) extracted and purified from salmon roe and milt sacs, a waste product of the fishing industry was studied for molecular binding and photoluminescence effects using bromocresol purple (BCP). Since BCP is both water and alcohol soluble it was investigated for binding efficiency in DNA/water solutions and modified DNA-CTMA/butanol solutions. Circular dichroism studies show that there is a maximum binding concentration of BCP in the DNA/water solution at ~5% by weight of BCP:DNA. In contrast, DNA-CTMA/butanol solutions showed increased binding concentrations up through 10wt% BCP:DNA-CTMA. This apparent binding affinity of DNA-CTMA for BCP also resulted in a significantly higher (6x) photoluminescence in thin film form when compared to BCP:PMMA films of the same doping concentration.
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Darnell E. Diggs, Darnell E. Diggs, Josh Hagen, Josh Hagen, Zhou Yu, Zhou Yu, Emily Heckman, Emily Heckman, F. Kenneth Hopkins, F. Kenneth Hopkins, James G. Grote, James G. Grote, Andrew Steckl, Andrew Steckl, "Molecular binding and enhanced photoluminescence of bromocresol purple in marine derived DNA", Proc. SPIE 5934, Nonlinear Optical Transmission and Multiphoton Processes in Organics III, 593407 (18 August 2005); doi: 10.1117/12.621954; https://doi.org/10.1117/12.621954
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