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29 September 2005 Selection of proper sensitizers for photodynamic therapy on the basis of time-resolved and steady-state photothermal study
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The possibility of application of natural photosynthetic pigments and their derivatives in photodynamic therapy and photodynamic diagnosis has been investigated. The triplet states of dyes are highly photochemically active, therefore, the dyes exhibiting high efficiency of triplet states generation are usually efficient photosensitizers in photodynamic therapy. Generation of triplet states has been estimated by means of the laser induced optoacoustic spectroscopy. The type of the photoreaction was established by comparing the yield of triplet generation with and without the oxygen presence. The intersystem crossing transition from excited singlet state S1 to triplet state T1 is competing with fluorescence emission, therefore, the dyes with efficient triplet generation usually are not suitable for diagnosis which requires a high yield of fluorescence. The chlorophyll-like pigments of prospective applications in medicine have been studied. Preliminary selection of dye-sensitizers has been made on the basis of investigation of the same dyes in model systems (solutions) and their complexes with macromolecules. The final study has been performed for resting (healthy) and stimulated (cancerous cells models) lymphocytes. Of course, the properties of sensitizers in cells and in models are not the same, but the measurements have enabled a selection (from a set of similar molecules) of the most promising dyes, tested later in the cells. The results obtained for chlorophyll-like pigments are compared with those obtained for the synthetic dyes (porphyrins, stilbazolium merocyanines and phthalocyanines) earlier investigated in our laboratory and with literature data.
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Danuta Frackowiak, Alina Dudkowiak, Ewa Staskowiak, and Krzysztof Wiktorowicz "Selection of proper sensitizers for photodynamic therapy on the basis of time-resolved and steady-state photothermal study", Proc. SPIE 5953, Acousto-optics and Photoacoustics, 595302 (29 September 2005);

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