23 September 2005 Vortex filter in light microscopy
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Abstract
We use a high resolution liquid crystal spatial light modulator (SLM) as phase modulator to generate different kinds of filters for light microscopy, placing it in a Fourier plane of the optical pathway. Manipulating light with a so called phase vortex filter can lead to an interesting kind of phase contrast imaging with remarkable properties. Using spatial coherent illumination from a laser diode, we observe strong edge enhancement within both phase and amplitude objects. The amplification is comparable to the Nomarski method, but with a higher degree of isotropy. The assembly can also provide an informative "shadow effect" (known as pseudo-relief) for shallow structures whose variations in thickness are smaller than the light wavelength. Furthermore, the method may be useful for an alternative kind of interferometric measurements, which solves an existing problem in conventional interferometry.
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Alexander Jesacher, Severin Fürhapter, Stefan Bernet, Monika Ritsch-Marte, "Vortex filter in light microscopy", Proc. SPIE 5959, Medical Imaging, 595907 (23 September 2005); doi: 10.1117/12.621835; https://doi.org/10.1117/12.621835
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