Towards optical characterization of biological media: analysis of backscattered images in linearly polarized light, simulations and experiments

Julie Falconet; Raphaël Sablong; Franck Jaillon; Emmanuel Perrin; Hervé Saint-Jalmes

doi:10.1117/12.621375

23 September 2005 Towards optical characterization of biological media: analysis of backscattered images in linearly polarized light, simulations and experiments

Julie Falconet, Raphaël Sablong, Franck Jaillon, Emmanuel Perrin, Hervé Saint-Jalmes

Author Affiliations +

Proceedings Volume 5959, Medical Imaging; 59590I (2005) https://doi.org/10.1117/12.621375
Event: Congress on Optics and Optoelectronics, 2005, Warsaw, Poland

Abstract

Optical imaging is a non invasive way to characterise turbid media, which is of real interest for investigating biological tissues for diagnosis purposes. A method called Integral Reflectance (IR) has already been developed [1]. The media being illuminated by a laser beam (670 nm, <1 mW), the backscattered light is captured by a 2D CCD camera. The reduced scattering coefficient μ's and the absorption coefficient μ_a are determined from the image. Having μ's and μ_a, the objective is to improve the characterization by estimating the anisotropy factor g, using polarized light. Different patterns depending on g are produced in these images, presenting some lobes, centred in the entry point of the laser beam, whose number and shape vary with g. To assess a simple description of these patterns, a circular outline of the image, at a given radius, is studied by Fourier series decomposition, namely Fourier descriptors, whose indices, modulus and phase provide the number, the size and the orientation of the lobes, respectively. Backscattered images of turbid media with g in the range [0.006 ; 0.93] (μ's = 10, 20, 40 cm^-1 ; μ_a = 0.01, 1, 5 cm^-1), were simulated using a Monte Carlo code for polarized light. Tables of Fourier descriptors were obtained as function of g, μ's and μ_a. Five reference solutions made of polystyrene spheres in liquid, with g varying from 0.71 to 0.919 (tissue phantoms) were tested. The Fourier descriptors were compared to simulations, and g could be retrieved with a maximum error of 10%.

Citation Download Citation

Julie Falconet, Raphaël Sablong, Franck Jaillon, Emmanuel Perrin, and Hervé Saint-Jalmes "Towards optical characterization of biological media: analysis of backscattered images in linearly polarized light, simulations and experiments", Proc. SPIE 5959, Medical Imaging, 59590I (23 September 2005); https://doi.org/10.1117/12.621375

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available