Novel approach to modeling of images emitted by a virtual oligonucleotide library

M. Boltrukiewicz; A. R. Cysewska-Sobusiak; J. Parzych

doi:10.1117/12.623181

23 September 2005 Novel approach to modeling of images emitted by a virtual oligonucleotide library

M. Boltrukiewicz, A. R. Cysewska-Sobusiak, J. Parzych

Proceedings Volume 5959, Medical Imaging; 59590Y (2005) https://doi.org/10.1117/12.623181
Event: Congress on Optics and Optoelectronics, 2005, Warsaw, Poland

Abstract

The molecular complementarity of two strands of the DNA double helix makes it possible to model changes in DNA sequences basing on their optical properties. The paper is devoted to problems with reliability of fluorescence analysis in investigating DNA chains. A given oligonucleotide library makes a matrix of integrated short sequences which all have their own location and base sequence. The fluorescence effects may be acquired using a CCD camera, converted to digital microimages and stored. In the paper the physical aspects of fluorescence phenomena have been described. The mathematical model for tests tending to determine optimum algorithms of oligonucleotide library detection are proposed. Application of selected algorithms of image processing has been analyzed for conditioning microimages. The results obtained of binarization using digital filtration are presented. The optimal algorithm of image binarization has been shown.

Citation Download Citation

M. Boltrukiewicz, A. R. Cysewska-Sobusiak, and J. Parzych "Novel approach to modeling of images emitted by a virtual oligonucleotide library", Proc. SPIE 5959, Medical Imaging, 59590Y (23 September 2005); https://doi.org/10.1117/12.623181

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KEYWORDS

Luminescence

Nonlinear filtering

Light sources

Digital filtering

Image filtering

Image processing

CCD cameras

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