Objective: To study the influence of 630nm semiconductor laser irradiation on rat ascetic tumor cells without photosensitizers. Methods: In this work, rat ascitic tumor cells (S180) were incubation with no photosensitizers. 630nm light (total output 2W) was delivered to tumor cells at different fluence rate: 100, 200, 250mw/cm2 . According to each fluence rate, S180 tumor cells were divided into 5 groups, which were irradiated with various time periods: 20, 15, 10, 5, 3min. In all cases, S180 tumor cells were taken photos. We use the morphology and flow cytometry technique to investigate the apoptosis of tumor cells during the experiment.
Results: 1. No obvious morphological difference between test groups and control groups at various time periods during the experiment. 2. No obvious cell apoptosis difference between test groups and control groups using FCM. Conclusion: After irradiated with 630nm semiconductor laser light, rat ascitic tumor cells (S180) with no photosensitizers incubation were not obviously killed or induced apoptosis.