12 October 2005 Synthesis and characterization of peptide nanostructures designed for sensing applications
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We report the design and the synthesis of membrane-active peptide nanostructures, as well as their use as signal transducer in a fluorimetric assay for biologically relevant analytes. Addition of hydrophobic 21-residue peptides bearing six crown ether side chains to a solution of small unilamellar vesicles loaded with a pH-sensitive fluorophore induces a rapid fluorescence increase associated with Na+/H+ transport across the bilayer membrane. To demonstrate the usefulness of these peptide nanostructures in the development of simple, rapid, and sensitive detection assays for a wide range of analytes, peptide nanostructures bearing a biotin at the N-terminal position were prepared. Addition of avidin to the assay employing these modified peptides resulted in a significant change in the time-dependent fluorescence profile. Control experiments with a non-binding proteins and saturated avidin showed that the observed changes are indeed due to specific binding of avidin to biotin modified nanostructures.
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Normand Voyer, Normand Voyer, Mathieu Arseneault, Mathieu Arseneault, Francois Otis, Francois Otis, } "Synthesis and characterization of peptide nanostructures designed for sensing applications", Proc. SPIE 5969, Photonic Applications in Biosensing and Imaging, 59690M (12 October 2005); doi: 10.1117/12.628791; https://doi.org/10.1117/12.628791

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