12 October 2005 Mass transport effects on real-time nucleic acid microarrays
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Microarray analysis has become increasingly complex due to the growing size of arrays. In this work we explore the effects of diffusion and convective fluxes on the time of hybridization and sensing specificity in a single component system. A .nite element software is used to simulate the diffusion of DNA through a microfluidic chamber to the sensing surface where hybridization of DNA is modeled using the corresponding kinetic equation. The differences between diffusion controlled and convection controlled mass transport are investigated as a function of concentration and hybridization time. Hybridization enhancement produced by microfluidics versus stationary diffusion is introduced as a useful metrics for quantitation of mass transport effects.
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Steve M. Blair, Steve M. Blair, J. A. Bishop, J. A. Bishop, A. M. Chagovetz, A. M. Chagovetz, "Mass transport effects on real-time nucleic acid microarrays", Proc. SPIE 5969, Photonic Applications in Biosensing and Imaging, 596910 (12 October 2005); doi: 10.1117/12.629413; https://doi.org/10.1117/12.629413

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