13 October 2005 Development of poly-l-lysine-coated calcium-alginate microspheres encapsulating fluorescein-labeled dextrans
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A technique to produce fluorescent cell phantom standards based on calcium alginate microspheres with encapsulated fluorescein-labeled dextrans is presented. An electrostatic ionotropic gelation method is used to create the microspheres which are then exposed to an encapsulation method using poly-l-lysine to trap the dextrans inside. Both procedures were examined in detail to find the optimal parameters producing cell phantoms meeting our requirements. Size distributions favoring 10-20 microns microspheres were obtained by varying the high voltage and needle size parameters. Typical size distributions of the samples were centered at 150 μm diameter. Neither the molecular weight nor the charge of the dextrans had a significant effect on their retention in the microspheres, though anionic dextrans were chosen to help in future capillary electrophoresis work. Increasing the exposure time of the microspheres to the poly-l-lysine solution decreased the leakage rates of fluorescein-labeled dextrans.
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Luc Charron, Luc Charron, Andrea Harmer, Andrea Harmer, Lothar Lilge, Lothar Lilge, "Development of poly-l-lysine-coated calcium-alginate microspheres encapsulating fluorescein-labeled dextrans", Proc. SPIE 5969, Photonic Applications in Biosensing and Imaging, 596924 (13 October 2005); doi: 10.1117/12.628831; https://doi.org/10.1117/12.628831

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