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30 September 2005 Towards an integrated coherent anti-Stokes Raman scattering (CARS) microscopy system
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Abstract
CARS microscopy has emerged as a powerful tool in imaging of biological matter. In addition to a high-3D spatial resolution, the technique delivers an attractive set of properties such as chemical specificity, high sensitivity, and fast data acquisition rates thus making it very suitable for biomedical applications. However, these advantages come at a cost of complex tunable laser sources, beam guiding and delivery optics. In particular, two high intensity laser pulses, whose carrier frequencies ωp (pump) and ωs (Stokes) are separated by the corresponding Raman shift value, are required to interact with the imaged media. In this paper, we present experimental results corresponding to our first step towards an integrated CARS-microscope. We demonstrate optical fiber delivery of two color picosecond pulses before they interact in the focus of microscope objective in order to produce CARS image. Certain aspects related to the effect of the pump and Stokes pulse parameters on image quality (e.g. contrast, sensitivity) after the pulses' propagation in the fiber will be addressed. The incorporation of fiber delivery feature significantly improves the microscope performance and ease its operation. In addition, we are exploring certain approaches in further development of CARS-microscope as a biomedical tool towards fully functional endoscope for in vivo chemically sensitive imaging.
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Francois Legare, Feruz Ganikhanov, and X. Sunney Xie "Towards an integrated coherent anti-Stokes Raman scattering (CARS) microscopy system", Proc. SPIE 5971, Photonic Applications in Nonlinear Optics, Nanophotonics, and Microwave Photonics, 597108 (30 September 2005); https://doi.org/10.1117/12.629926
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