Cellular vitality of PC12 cells revealed through auto-fluorescence induced by two-photon excitation

Elena Perevedentseva; Artashes Karmenyan; Fu-Jen Kao

doi:10.1117/12.640220

7 December 2006 Cellular vitality of PC12 cells revealed through auto-fluorescence induced by two-photon excitation

Elena Perevedentseva, Artashes Karmenyan, Fu-Jen Kao

Proceedings Volume 5973, Current Research on Laser Use in Oncology: 2000-2004; 597311 (2006) https://doi.org/10.1117/12.640220
Event: Current Research on Laser Use in Oncology: 2000-2004, 2004, Moscow, Russian Federation

Abstract

The combination of fluorescence spectroscopy and optical trapping under an integrated laser scanning microscopy platform has presented to be a powerful tool in manipulating and positioning the cells as well as standardizing the conditions of measurements. This platform was applied to investigate the autofluorescence of PC12 cells. Under 2-p excitation, the PC12 cells exhibit fluorescence in the range of 500-530 nm. It was shown that intensity of 2-p autofluorescence from PC12 cells is very sensitive to the cells' condition. Therefore autofluorescence from the cells may be a convenient and noninvasive method for monitoring the cell's or another biological object's condition, metabolism, and/or vitality under various physical and chemical influences.

Citation Download Citation

Elena Perevedentseva, Artashes Karmenyan, and Fu-Jen Kao "Cellular vitality of PC12 cells revealed through auto-fluorescence induced by two-photon excitation", Proc. SPIE 5973, Current Research on Laser Use in Oncology: 2000-2004, 597311 (7 December 2006); https://doi.org/10.1117/12.640220

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available