5 November 2005 THz absorption signature detection of genetic material of E. coli and B. subtilis
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Proceedings Volume 5995, Chemical and Biological Standoff Detection III; 59950N (2005); doi: 10.1117/12.629959
Event: Optics East 2005, 2005, Boston, MA, United States
Abstract
The development of efficient biological agent detection techniques requires in-depth understanding of THz absorption spectral features of different cell components. Chromosomal DNA, RNAs, proteins, bacterial cell wall, proteinaceous coat might be essential for bacterial cells and spores THz signature. As a first step, the DNA's contribution into entire cell THz spectra was analyzed. The experimental study of cells and DNAs of E. coli and cells/spores and DNA of Bacillus subtilis was conducted. Samples were prepared in the form of water solutions (suspension) with the concentrations in the range 0.01-1 mg/ml. The measurable difference in the THz transmission spectra of E. coli and Bacillus subtilis DNAs was observed. The correlation between chromosomal DNA signature and a corresponding entire spore/cell signature was observed. This correlation was especially pronounced for spores of Bacillus subtilis and their DNA. These experimental results justify our approach to develop a model for THz signatures of biological simulants and agents. In parallel with the experimental study, for the first time, the computer modeling and simulation of chromosome DNAs of E. coli and Bacillus subtilis was performed and their THz signatures were calculated. The DNA structures were optimized using the Amber software package. Also, we developed the initial model of the DNA fragment poly(dAT)-poly(dTA) solvated in water to be used in the simulations of genetic material (DNA and RNA) of spores and cells. Molecular dynamical simulations were conducted using explicit solvent (3-point TIP3P water) and implicit solvent (generalized Born) models. The calculated THz signatures of E. coli and Bacillus subtilis DNAs and poly(dAT)-poly(dTA) reproduce many features of our measured spectra. The results of this study demonstrate that THz Fourier transform infrared spectroscopy is a promising tool in generating spectral data for complex biological objects such as bacterial cells and spores.
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Alexei Bykhovski, Xiaowei Li, Tatiana Globus, Tatyana Khromova, Boris Gelmont, Dwight Woolard, Alan C. Samuels, James O. Jensen, "THz absorption signature detection of genetic material of E. coli and B. subtilis", Proc. SPIE 5995, Chemical and Biological Standoff Detection III, 59950N (5 November 2005); doi: 10.1117/12.629959; https://doi.org/10.1117/12.629959
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KEYWORDS
Terahertz radiation

Absorption

Genetics

Biological weapons

Proteins

Spectroscopy

Biological research

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