8 November 2005 Fluorescence coupling into structured waveguide as platform for optical portable sensors
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Abstract
Optical chemical sensors and biosensors are attracting research interest in applications such as environmental monitoring and biomedical diagnostics. Structured Integrated Optical Waveguide is one solution to reduce the reader's cost and size. The principle is the capture of fluorescence emitted by Qdots at the surface of a rib waveguide, which collects then guides it at the end-face of the chip to be detected. However, fluorescence coupling into a waveguide is still not easy to predict as it depends on fluorophore's environment and dipole's orientation and location. We report here the validation of a simple theory concerning optimization of optical waveguide's thickness considering a fluorophore's position. Optimisation of coupling power between a dipole and a guided mode can be simplified by the optimisation of the guided mode's intensity ratio integrated in the 5 nm region over the guide's core surface (where QDots are supposed to settle) divided by the whole guided intensity. A model has been developed from the work of Marcuse1: coupled power is proportional to the square of the electrical field of the guided wave. As a result, this model gives an optimal core's thickness and efficiency of coupling depends on polarisation. Moreover, FDTD simulations do complete this study. Three thicknesses have been therefore experimentally deposited: 100 nm, 125 nm and 150 nm. To conclude, experimentation corresponds to the model. A new, sensitive and potentially low cost portable transducer for the analysis of all kinds of biomolecular affinity systems has been developed and validated.
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Anne-Laure Seiler, Anne-Laure Seiler, Pierre Labeye, Pierre Labeye, Patrick Pouteau, Patrick Pouteau, Frédéric Mallard, Frédéric Mallard, Xavier Hugon, Xavier Hugon, Pierre Benech, Pierre Benech, } "Fluorescence coupling into structured waveguide as platform for optical portable sensors", Proc. SPIE 5996, Optical Sensors and Sensing Systems for Natural Resources and Food Safety and Quality, 59960H (8 November 2005); doi: 10.1117/12.631161; https://doi.org/10.1117/12.631161
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